Sequence comparison showed that the replicon of pBMB165 was homologous to the pAMβ1 family replicons, indicating that the pBMB165 replicon belongs to this family.
This highly species-specific distribution of replicons indicate a narrow host range profile of the Rep_3 family.
Haloarchaeal genomes are normally composed of multiple replicons (chromosome, minichromosome, and plasmids) with multiple Orc/Cdc6 homologs (usually more than 10 homologs) [ 19- 30], indicating that the occurrence of multiple replication origins is widespread in haloarchaea.
However, the existing strategies for the de novo construction of GGL HBV replicons are too complex to implement, especially when multiple replicons need to be cloned.
Bacteria harboring multiple replicons inside and outside the Burkholderia genus have also been shown to have a distributional bias in their conserved or induced genes.
The origin and function of multiple replicons in bacteria is just beginning to be studied; however, studying these organisms may give insight into the origin of multiple chromosomes in higher organisms.
Overlapping predicted loci conserved in multiple replicons were parsed into a single locus; the reported E value and score for each locus corresponds to the lowest E value and highest score of these overlapping candidates.
Haloarchaeal genomes are generally composed of multiple replicons, and each replicon has a single or multiple replication origin(s).
Multiple replicons may have arisen from the need to achieve higher overall replication rates [ 19].
Multiple replicons were identified in 10 Azospirillum species as with B510.
