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This novel distribution of J2 was reproducible in multiple replicates with independently hatched nematodes.
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All experiments were independently repeated three times, with multiple replicates within each run.
Another advantage is that multiple replicates are available with identical conditions for each new experiment.
The RILs were planted in a simple lattice design with multiple replicates in a plot at Jiangpu Station, Nanjing Agricultural University, Nanjing, China.
MATS in particular, allows differential splicing analyses with multiple replicates.
Given these three parameters, we can simulate gene expression levels in two conditions with multiple replicates.
Table 1 shows two representative genes with multiple replicates for the expression ratios of Fum/O2.
Linear regression with repeated measures was used to analyse IRGM expression (as estimated by ΔCt values from Q-RT PCR) with multiple replicates for each individual.
The net absorbance (A450−A650) was used to determine the percentage of viable cells relative to controls: 2 All experiments were carried out with multiple replicates as specified elsewhere.
All experiments were performed two to three times, with multiple replicates at each time point (total of n = 4 to 6 independent samples).
In addition, our simulations suggest that our PSG method can produce more accurate DP predictions than those from Cuffdiff and FDM, even when these methods are provided with multiple replicates per condition.
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