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Amplification, probe preparation and hybridization protocols were performed as previously described [9] and multiple replicates were examined in each condition.
Multiple replicates were compiled for statistical analysis and presentation.
At the time we sequenced the transcriptomes, costs associated with sequencing multiple replicates were prohibitive.
Where raw data of multiple replicates were available to us [ 12], noise variance was quantified directly.
For each experimental condition, a total of five to ten random fields from multiple replicates were taken at magnification X200.
Additionally, for every experimental condition for which multiple replicates were available, a median M and A value across replicates was calculated.
Similar(53)
Another advantage is that multiple replicates are available with identical conditions for each new experiment.
The model for data originating from multiple replicates is illustrated in Figure 3.
A simple statistical method for estimating variances from microarray control data which does not require multiple replicates is presented.
Technical replicates are used to establish experimental variability in NGS procedure (Robasky et al., 2013), though multiple replicates are not necessary for RVD2.
Grapevine, being a woody perennial crop, is challenging to use in controlled conditions experiments, especially where large amounts of material and multiple replicates are required.
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