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To facilitate quantitative sampling of the viral population, cDNA generation from viral RNA and subsequent PCR reactions used high template volume, low cycle numbers, and multiple replicates that were pooled for sequencing (Fig. S5).
To allow quantitative assessment of the viral population, both cDNA generation from viral RNA and subsequent PCR reactions used high template volume, low cycle numbers, and multiple replicates that were subsequently pooled for sequencing (Fig. S5).
Comparative quantification was carried out using multiple replicates that were analysed in triplicate.
Application of these analytic methods requires multiple replicates that are prohibitively expensive with the ChIP-chip experiments.
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We present results from multiple replicate screens that highlight several extracts capable of significantly altering insulin and pdx1 promoter activity in beta-cells.
These authors concluded that more reliable analyses of gene expression data are obtained by pooling data from multiple replicates, and recommended that at least three replicate hybridisations be completed for each experimental treatment.
As is still the case with many deep sequencing datasets, we do not have multiple replicates at our disposition that can be used to identify significantly differing APAs across tissues.
Another advantage is that multiple replicates are available with identical conditions for each new experiment.
We performed lifespan analyses on over six thousand isp-1 qm150 isp-1 qm150FPgst-4 GFPol Pgst-4 GFP andmals that encontroled multiPgst-4 GFPtes Pgst-4 GFPanimalsthatd on thencompassedifferent transcription factor RNAi clones.
A custom designed miRNA microarray that incorporated multiple replicates of 3,098 unique probes for sheep and other mammalian miRNA was used.
Joint likelihoods for multiple replicates are obtained by assuming that the replicates are independent conditional on the genotypes of all contributors and parameters ϕ such as the amounts and degradation levels of DNA from each contributor [2].
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