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An additional precaution against false negatives is resequencing multiple replicates from the same experimental setup.
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4) Is the read density data in Figure 1 figure supplement 1 reporting a single representative sample, total summed reads from replicates (if done) or mean reads from multiple replicates?
The results presented herein are from single experiments representative of the findings obtained from multiple replicates.
Data from multiple replicates of the same day 0 sample were averaged to provide a robust "time-zero" baseline.
Right panel: western-blot quantification from multiple replicates.
We combined values from multiple replicates of the same experiment by taking their average.
All data are expressed as means ± S.E.M. of values from multiple replicates per group.
All experiments were repeated several times, and in each experiment data were collected from multiple replicates.
All experiments were repeated several times and in each experiment data were generated from multiple replicates.
Right panel: western-blot quantification for the normalized 3B5H10 signals from multiple replicates.
The model for data originating from multiple replicates is illustrated in Figure 3.
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