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The net absorbance (A450−A650) was used to determine the percentage of viable cells relative to controls: 2 All experiments were carried out with multiple replicates as specified elsewhere.
Thus, a quantitative analysis requires normalization and averaging of multiple replicates (as we do in Figure 4B).
In Figure 1C, we plot the mean divergence between the true and estimated admixture proportions, over multiple replicates, as a function of resolvability.
Joining genes from common functional groups is effectively the same as using multiple replicates as it dramatically increases the power of the experiment.
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It will be essential for researchers working in new systems to ascertain good material production pipelines, with the capacity to perform multiple biological replicates, as well as organ, tissue, and cell-type dissections and mass isolations (see below).
We highlight, however, that any candidate genes identified in this study (by SSH or transcriptomic analysis) should be subjected to rigorous evaluation on a gene-by-gene basis in controlled and natural re-infection experiments with multiple biological replicates, as well as in functional experiments, before their role(s) in Symbiodinium-symbioses can be confirmed and delineated.
Since MochiView can display multiple data sets on the same y-axis, the user can easily overlay multiple replicates of experimental ChIP data as well as control data sets (for example, ChIP in a deletion or RNAi-depleted strain or in a strain lacking the epitope tag targeted for immunoprecipitation).
They find that NuGEN performs remarkably well across multiple laboratories and across technical replicates as low as 500 pg [ 28].
Simple steps to reduce noise such as averaging multiple replicates, or to increase signal such as increasing heart rate, can improve information content, and therefore viability, of any optimization process.
The average P values across multiple replicates (1,000) were used as the P value for that site.
Five thousand Calu-1 cells were seeded as multiple replicates in a 96-well plate and the next day treated with 5 μmol/L curcumin for 72 h.
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