In this study, the infiltration capacity of six common materials was tested by multiple replicate experiments with automated mini-disk infiltrometers.
Error bars presented in the figures represent standard deviations of the means of multiple replicate experiments.
The method takes as input log2 SILAC protein ratio values, corresponding to relative abundances, for 1 or multiple replicate experiments.
We then proposed multivariate statistics that integrate the results from multiple replicate experiments to compute false discovery rates, the total number of differentally abundant proteins, and statistical power.
We further extended the method of Efron to facilitate its application to quantitative proteomics experiments by developing multivariate statistics that integrate the results of experiment-specific models of multiple replicate experiments to compute false discovery rates, statistical power, and the total number of differentially abundant proteins.
In summary, our method models log2 SILAC protein ratio values from 1 or multiple replicate experiments and infers the full shape of class-conditional probability distributions for biologically relevant proteins (i.e. those that differ in abundance between the 2 samples) versus background.
from multiple replicate experiments.
Although some variation was noted in cells from different donors, the experiments described were very reproducible in multiple replicate experiments, utilizing several human donors.
In contrast to drug-sensitive A2780 cells, however, although puromycin-resistant colonies were initially formed in A2780DPP cells in multiple replicate experiments, the colonies formed were relatively small (10 20 cells per colony) and were viable for only 48 h.
Finally, replication is a key consideration in any experiment, and especially in experimental evolution, where random genetic drift plays a large role: observation of a large allele frequency difference at a locus in multiple replicate experiments will increase confidence that the difference is due to selection at the locus rather than drift.
