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The analysis addressed age-independent differences among the selective regimes using multiple replicate lines per treatment.
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This particular CIM mapping program was chosen as it has provision for appropriate analysis of multiple replicates of each line and for multiple environments (+UV-B and -UV-B, in our case) [ 64].
Replicate lines of Biomphalaria glabrata were set up consisting of snails either previously artificially-selected over multiple generations for increased resistance to Schistosoma mansoni infection (R-lines), or unselected susceptible lines (U-lines), or mixed 'populations' of both resistant-selected and unselected host genotypes together (R U-lines).
Multiple replicate chips were printed, each carrying all six conditions printed in multiple replicate spots.
Multiple replicate analyses of serum pool aliquots were performed.
from multiple replicate experiments.
Multiple replicate copies of the library were thus generated.
For each experimental condition, we used six replicate lines.
Replicate lines were randomly assigned to two treatments.
The pancreatic line has a large number of states, but relatively few replicates, while the motor neuron line has multiple replicates per state.
One lineage remained unclassified because multiple replicates failed to grow.
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