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Reproducibility of the analysis was tested by running multiple repeat experiments (duplicate or triplicate analyses of identical strains).
Parametric analysis of combined data from multiple repeat experiments, or experiments containing more than two groups, was performed using ANOVA, followed by Tukey HSD post-hoc tests.
However, in multiple repeat experiments normalized to protein expression changes, cells expressing iKR PR consistently exhibited reduced PARP cleavage relative to cells expressing iWT PR.
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Among these, cell culture offers the advantages of availability of greater number of cells and facility to perform multiple and repeated experiments over long time periods.
Reported rates and the associated errors are reported as averages and standard deviations determined from fits to traces acquired in multiple repeat stopped-flow mixing experiments.
The clustering of wounds on the basis of their healing states was broadly consistent across the multiple groups used in repeat experiments (see Figure S-11 in the Supporting Information).
However, it appears that ecologists still only rarely repeat experiments across multiple years (Vaughn and Young 2010).
Furthermore, we can use the multiple repeats of the experiments to evaluate the statistical significance (p-value) of these eSDC values, i.e. the probability that we would obtain the value due to noise in the data.
It must be noted that in the multiple repeats of these experiments, there were occasions when the E6 protein alone was seen to slightly reduce E-cadherin protein level.
For transcriptome analysis involving a large numbers of samples such as in a time-course experiment with multiple repeat, it is economical to use DNA microarray-based technologies.
Although the amount of 14-3-3 14-3-3 14-3-3ghtly reduced in the Chk1 knockdown in Fig. 1D, this was not observed in multiple repeats of the experiment, including Fig. 1F, and was thus likely a blotting defect.
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