A U.S. court ordered Google to remove the film, but it had refused to cave in to multiple removal requests in Pakistan, where the film incited deadly protests and — ultimately — a full block on YouTube.
The sites cooperated, but, she said, "there is still a ghost presence on Google," even though she sent it multiple removal requests.
Standard data processing was applied with sampling rate of 2 ms, including trace editing, deconvolution, multiple removal, NMO correction with stretching factor of 1.5, CDP stacking and post-stack time migration with maximum dip of 60 degree; these methods are described in detail by Yilmaz and Doherty (1987).
To reduce non-specific reactions and gain a higher sensitivity, high molecular weight proteins contained in the sera were removed by Multiple Affinity Removal Spin Cartridge Reagent Kit (Agilent Technologies, Santa Clara, CA, USA) and used for the serum samples.
But both these approaches become more involved and complex for multiple column removals.
Here we demonstrate the capabilities of these new features using the mitochondrial atp6 gene of Isoetes engelmannii since this extremely edited locus combines the requirements of introducing start and stop codons with multiple stop removals of in-frame stops.
Serum samples were processed using the Agilent Multiple Affinity Removal Column (Multiple Affinity Removal Column, 4.6 mm × 50 mm; Agilent Technologies, Palo Alto, CA), that selectively removes albumin, IgG, IgA, antitrypsin, transferrin and haptoglobin from the serum sample.
EDTA was added at sample collection time, protease inhibitors were later added and high abundance proteins were removed using the Agilent Multiple Affinity Removal System described above.
Consequently, we used a High Capacity Multiple Affinity Removal Spin Cartridge to remove 14 abundant proteins from SF. Healthy pooled samples (n = 6) were labeled with O and individual samples from early (n = 5) and late (n = 4) OA were labeled with O as described in the Methods section.
Applied methods (Multiple Affinity Removal System, Agilent Technologies, Inc). enables removal of 7 or 14 most abundant plasma proteins.
