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Recent phylogeographic and biogeographic studies have uncovered multiple refugia in Iberia (see [ 16] for a review).
This highlights the importance of multiple refugia in mountains as sources of contemporary genetic variation in alpine species.
The phylogeographic structure of Narceus reveals a complex evolutionary history with signatures of multiple refugia in southeastern North America followed by two major northern expansions.
Present results confirm the Iberian origin of the land snail E. quimperiana and strongly support the emerging phylogeographic hypothesis of multiple refugia in Iberia during the last glaciations.
In agreement with this and other studies e.g.[ 46], a scenario of multiple refugia in mountains adjacent to the Central Pyrenees (i.e., the southern mountains of the pre-Pyrenees) is gaining more support (see Figure 3).
This pattern of multiple refugia in the Balkans was already erected by Reinig [ 39] postulating different centres of survival in the western, southern and eastern Balkans and was later supported by genetic analyses showing genetic divergences between these areas for a variety of different animal species [e.g. [ 18, 40- 42]].
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These are the first data to suggest multiple refugia existed in the Sierra Nevada, and on both sides of the crest, during glacial phases.
Glacial cycles may result in fragmentation of a widespread ancestral species into single or multiple refugia [27], [28], resulting in genetic divergence among small isolated populations, especially in sky islands, hence driving speciation (reviewed in [29]).
If R. b. balteatus did survive the Pleistocene in multiple refugia, as our data suggest, the locations appear to be in the lower Columbia River system and/or along the Pacific Coast, and somewhere in the Upper Columbia River drainage, as noted above.
However, our analyses of diversification pattern in G. elliotii appear to reflect historical differentiation in multiple refugia.
A recent phylogeographic analysis of cpDNA variations in S. alpina indicated that this alpine shrub survived in multiple refugia during the Last Glacial Maximum and that earlier glaciations may have triggered deep intraspecific divergence (Zhang et al., 2012).
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