Sentence examples for multiple reference genes from inspiring English sources

To minimize this innate variability, multiple reference genes are used.

However, multiple reference genes showed higher expression stabilities and higher reliability.

However, other researches have reported that multiple reference genes could increase instability and experimental complexity (Kong et al. 2015; Li et al. 2015).

Utilization of multiple reference genes is a way to relieve the effect of expression variability of single reference genes (Vandesompele et al. 2002).

However, some researchers (Kong et al. 2015; Li et al. 2015) have indicated that multiple reference genes could increase instability and experimental complexity.

In the current study, among the 5 traditional reference genes B-TUB11, B-TUB2, Rps-24, UBC, and α-ACTIN) tested, UBC was identified as a stable expressed gene (Cho et al. 2014); however, multiple reference genes have been reported in other studies (Kong et al. 2015).

Multiple reference gene normalization was chosen.

EF1 and RpSA3 were used as reference targets for the multiple reference gene normalization approach as implemented in qBasePLUS2 software (Biogazelle).

The use of one or multiple reference gene(s) in the calculation of relative expression data can have a significant influence on the final normalized results.

PCR reactions were performed in duplicate and gene expression levels were determined using iQ5 software (Biorad) using a Δ-Cq relative quantification model with PCR efficiency correction and multiple reference gene normalization [ 43, 44].

To generate a more robust method for standardised determination of the genomic content of cfDNA, this study applied the widely accepted best practice principle for gene expression analysis of multiple reference gene normalisation [ 46, 56] (Fig.  7).