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Multiple reads of a single file must be able to complete without waiting for one another.
Consensus sequence from multiple reads of individual samples were generated from the trace files through base calling and multiple sequence alignment (MSA) by phred [21], phrap [22] and MACE-multiple alignment consed extensions (Gilliland & Langley, University of California, Davis), and visualized by consed [23].
Multiple reads of both the mature and star miRNA sequences (typical components of a miRNA) were found in this library.
For instance, coupling with nuclear spins has been used both to allow multiple reads of the electron spin state(269) and to read out nuclear spins.
Typically each position of DNA sequence is covered by multiple reads of a short read dataset and our algorithm makes use of resulting redundancy to achieve high compression rate.
Since most of our candidates were supported by a single read, one simple filtering criterion was to consider only fusions supported by multiple reads, of which we found 19 (Table 2).
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In the sequences with multiple reads, 60% of them were sequences with 10 reads or less.
It was shown that 72.7% of the 33-mer sequences were just single read, 24.4% of those had multiple reads, and 2.9% of unique sequences were low quality sequences containing at least one unknown nucleotide 'N'.
Dividing PUTs consisting of multiple reads (i.e. the isotigs) by the origin of their reads is a simple, but useful method to identify potential treatment-specific sequences.
However we greatly improved the percentage of uniquely mapped reads from 22%to45%5% while decreasing the number of multiple reads from 58%to32%2%.
Even after multiple reads over the course of my life – five?
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com