Exact(3)
The analog signal readout is performed by multiplexing in the current mode independent of the signal charge collection enabling multiple readout cycles with negligible dead time and thus imaging with wide dynamic range.
This includes target risk assessment, biomarker evaluation and predictivity grading both for efficacy and toxicity, early human trial design adequate to guide stop/go decisions on grounds of biomarker panels, and biostatistical methods to analyze multiple readout situations and quantify risk projections.
The gene-by-gene high-throughput screening assay was designed using a robotic pipetting system for siRNA transfection, optimized in a 96-well format, with multiple readout options for measuring GFP expression.
Similar(57)
To calculate the short-term and long-term memory in the combined activities of multiple signals, we took the same approach, with the one difference being that the clustering step captured the combined behavior of multiple readouts (Step 3 in the algorithm in Materials and Methods).
The 96-well format, and multiple readouts of host damage and metabolic alterations (through luminescence, chlorophyll fluorescence and Sytox Green® fluorescence indicative of membrane damage, all of which are quantitative measures) makes this an excellent system amenable to a wide variety of automation assisted high-throughput screens.
To demonstrate the effect of treatment on multiple readouts, we performed a histopathological analysis on mBSA-challenged paws from mice treated with anti-TNFα.
In addition, blocking TNFα leads to effects on multiple readouts, both systemic and local, including serum biomarkers also useful as biomarkers in RA: MMP3 and acute-phase proteins.
Third, traditional techniques are applicable to a wider range of assays, from easy ligand-binding bioassays to high-content cellular biological assays with multiple readouts.
This resulted in improved in vivo sensitivity and stability characteristics of optical reporters that enabled multiple readouts and (development of) high-throughput assays.
The availability of overlapping segments also provides multiple readouts for many of the residues, providing greater confidence in the rate measurements.
Multiplexing approaches are advantageous for a variety of reasons including, (1) the ability to undertake combinatorial biological approaches where different reporter readouts allow the association of different biological events, (2) multiple FP readouts can further resolve molecular mechanisms, and (3) data can be quickly acquired from multiple readouts in the same sample.
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