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Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models.
Robust genes that identify rejection within multiple rat strains might be more likely to serve as reliable biomarkers in patients with their inherent heterogeneity.
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In fact, our analyses across multiple inbred rat strains that are models of hypertension, obesity, and dyslipidemia found no genes in common between all disease strains [ 13].
Rat strains were derived from multiple founder colonies and, moreover, rat strains derived from the same outbred founder colony were derived at different geographical locations where subsets of the founder colonies were distributed and then maintained.
From these disease portals, we identified rat strains associated with multiple portals.
Multiple factors contribute to susceptibility/resistance of different rat strains to AA. Foremost among these is the MHC haplotype (for example, LEW rats (RT.1l) versus BN rats (RT.1n)).
The evolutionary history of laboratory rat strains is complex, as they were derived by multiple rounds of interbreeding and inbreeding in different locations at different points in time (Jong, 1984; Saar et al., 2008).
The purpose of this study was to define, both qualitatively and quantitatively, the manner in which the mammary glands of susceptible ACI and resistant BN rats respond to E2. Dramatic differences in multiple cellular and molecular responses to E2 were observed when these two inbred rat strains were compared.
We have all these incredibly valuable rat strains that represent particular models of human disease, like hypertension or heart disease.
(3) Reduce call blocking/dropping probability through multiple RAT selection.
Reduce call blocking/dropping probability through multiple RAT selection.
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