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The CBA technology provides the ability to quantify multiple proteins within a given sample, with precision and consistency.
These properties make the pEAQ vectors particularly suitable for use in situations, such as the investigation and manipulation of metabolic pathways, where the coexpression of multiple proteins within a cell is required.
Here using fluorescence molecular tomography (FMT), we present a non-destructive and versatile approach to quantify adsorption of multiple proteins within 3D environments and reveal the dynamics of adsorption of human serum albumin (HSA) and fibrinogen (Fib) on 3D polymeric scaffold.
Changes in the expression level of multiple proteins within a pathway increase the confidence that the differential expression of a single protein using traditional statistical methods is likely to be associated with the studied condition [19].
Many biomedical applications rely on the ability to co-express multiple proteins within the same cell.
The introduction of three primary biosynthetic activities to cells requires the stable coexpression of multiple proteins within the same cell.
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Towards this goal, (mini- cellulosomes have been developed within S. cerevisiae both as consortia, where enzymini- cellulosomesroteins are grown in individual strains [ 118], or as multiple proteins expressed withavea single strain [ 119].
In order to validate 2-DE-based proteomic data and/or to discriminate between the multiple proteins identified within the same differentially represented spot, cerebella samples were also subjected to two-dimensional western blotting analyses with specific antibodies (Supplementary Figure 3).
Since multiple proteins often occur within a sentence (∼40% in the dataset), a small window of context would cause less ambiguity.
Cytokine and matrix metalloproteinase levels were measured using an addressable laser bead multiplex assay (BMD, Paris, France) on a Luminex S100 analyser (Luminex Corporation, Austin, TX, USA); multiple proteins were detected simultaneously within a serum sample (Stebbing et al, 2008; Bower et al, 2009).
Second, protein translations of both the 5'- and 3'-fractions of a split CDS may cluster within the same OG, giving rise to multiple proteins from the same genome within a given OG (i.e., apparent paralogs).
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