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As one of the earliest examples of alternative gene splicing, which enables a single gene to produce multiple proteins, the discovery made a splash when it was published in Nature in 1982.
The assembly of the preRC involves the stepwise recruitment of multiple proteins, the nucleation of which begins with the arrival of the Origin Recognition Complex (ORC) [2].
If a subset of peptides was matched to multiple proteins, the protein that was supported by the most peptides over the eight mass spectrometric measurements was selected as the representative.
The envelope of ODV is not well characterized; and although it may be composed of multiple proteins, the mechanism by which it facilitates the initiation of infection of insect midgut cells is unclear [reviewed in [ 2]].
Studies were performed on complexes with only two members as well as larger systems with multiple proteins; the proteins studied came from all types of organisms including human, yeast, E. coli, cyanobacteria, or other microorganisms.
Since one spot could potentially represent multiple proteins, the best fit protein for each spot was determined by selecting the best percentage of confidence (≥99%) for the peptide assignation and best coverage of the protein using the protein software Scaffold (Proteome Software Inc., Portland, OR).
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Multiple proteins not involved in the signal transduction cascade have been identified which stabilize receptor surface expression [ 34].
The immunoblots were stripped in Restore Western blot stripping buffer and reprobed to assess the multiple proteins in the same blot.
First, there are limitations on proteome resolution, leading to the co-migration of multiple proteins to the same location on the gel.
The initiation of MTS affects the expression of multiple proteins.[22] [24] The identification of proteins that are characteristic of metastasis might allow the discrimination of different tumor entities.
The substitution rates thus increase in multiple proteins in the electron transport chain following the divergence of anthropoid primates from tarsiers.
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