In contrast to the RBFOX1/2 decoy oligonucleotides, which showed specific binding to RBFOX1/2 proteins in the proteomic analysis, the SRSF1 decoy oligonucleotides bound multiple proteins in addition to SRSF1.
The chicken-specific techniques will be of highest interest to investigators using avian embryos, development and packaging of lentiviral vectors that reliably express multiple proteins in infected cells should be of interest to all investigators whose experiments demand manipulation and expression of multiple proteins in developing cells and tissues.
In the frame of the NANOSPAD FP6 European project, a novel compact instrument capable of ultra-sensitive analysis of multiple proteins in a customized microarray has been designed and implemented.
The 2A oligopeptide is emerging as a highly effective new tool for the facile co-expression of multiple proteins in a single transformation step, whereby a gene encoding multiple proteins, linked by 2A sequences, is transcribed from a single promoter.
Over the past several years, we have taken advantage of the large capacity of these vectors to explore the expression characteristics of several dual promoter and 2A peptide bicistronic transgenes in developing chick neural retina, with the goal of identifying transgene designs that reliably express multiple proteins in infected cells.
Thus tracking multiple proteins in parallel or additional morphological identification must be carried out for confirmation.
It is thus possible to implement multiplex iLAMP assays for detecting multiple proteins in a sample.
These results suggest that RACK1A functions in rice innate immunity by interacting with multiple proteins in the OsRac1 immune complex.
As a result, microfluidics chips that can analyze multiple proteins in blood have been developed to save time and resources.
The mutual orthogonality of the SNAP- and CLIP-tags enables the simultaneous labeling of multiple proteins in the same cellular context.
The substitution rates thus increase in multiple proteins in the electron transport chain following the divergence of anthropoid primates from tarsiers.
