Preliminary experiments were performed using multiple proteins to demonstrate inclusion of structure during immobilisation.
Multiple sequence alignment of these proteins demonstrates the presence of strong sequence conservation at the location of the SSF46785 domain across all proteins, including DVU0151 (Additional file 3).
However, our data demonstrate multiple proteins whose abundance is also 'cell cycle regulated' at other stages during interphase, outside of mitosis, which suggests that they should also be annotated with the GO term 'cell cycle'.
Recent proteomics analyses have demonstrated that multiple proteins are in complex with these proteins; therefore, there may be additional proteins that directly associate with the complex for the regulation of the unwinding activity of SNRNP200 15, 15.
A moonlighting protein demonstrates multiple autonomous and usually unrelated functions.
The evidence presented here, indicating that the three proteins have tight interaction with multiple proteins involved in gastrin signaling, demonstrates that our approach is well suited to identify testable hypotheses, even for candidates for which knowledge in public databases is still sparse.
Application of iASeq to 77 ChIP-seq samples from 40 ENCODE datasets and 1 genomic DNA sample in GM12878 cells reveals that allele-specificity of multiple proteins are highly correlated, and demonstrates the ability of iASeq to improve allelic inference compared to analyzing each individual dataset separately.
In the current work, we observed increased nitrotyrosylation in multiple proteins of LA-treated HM cells (Figure 2), demonstrating that the LA responsive peroxynitrite generation enhanced nitrosative stress.
Thus, our findings, together with the work of other groups, demonstrate that miR-124 may target multiple proteins that function spatiotemporally or in cooperation with different cellular processes.
The results demonstrated that it is possible to purify multiple proteins from untreated crude resource with a rationally designed expanded bed adsorption process.
