Exact(2)
Multiple proteins co-purified with DGAT1 fusion protein.
Silver staining of the purified fractions indicated multiple proteins co-purified with the recombinant protein (data not shown).
Similar(58)
Interestingly, consistent with the observation that LDs intimately associate with the inclusion, a subset of inclusion membrane proteins co-purified with LD protein extracts.
In addition, since all the proteins were purified from yeast cells, we cannot exclude the possibility that interacting proteins co-purified with the tagged protein nominally present at a spot.
A number of cellular proteins co-purified with OSF (One-STrEP-FLAG -tagged NEDD4L, as visualized by SDS-PAGE.
The quantification of the H L ratio of proteins co-purified with the bait protein allows the relative quantification of recovered proteins.
As FLAG Gαq purified from Hepa-1 cells overexpressing the protein co-purified with endogenous Gβ and purified FLAG-Gβ1 co-purified with endogenous Gαq/11, we next tried to clarify which subunit is responsible for the lysoPLD activity we observed.
These histidine-rich proteins co-purify during IMAC procedures rendering recombinant protein preparations impure.
Indeed, SDS-PAGE analysis of "purified" fetuin-A preparation (Calbiochem, Cat. No. #341506) revealed multiple contaminating proteins that co-purified with the typical fetuin-A "doublet" bands on SDS-PAGE gels (Fig. 4A).
Among the proteins which co-purified with multiple Ccr4-Not subunits and which were not previously reported were the S-adenosylmethionine synthetases, Sam1 and Sam2, ( 1, Table S1, Figure S1), enzymes that regulate the cellular pool of S-adenosylmethionine (SAM) which is the universal methyl donor required for numerous biochemical reactions including Hmt1-dependent protein methylation [50].
In addition, three ribosomal proteins were co-purified with Nop1; ribosomal proteins are common false positives in protein complex purification procedures (Table 1)[ 31].
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