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In the multiple proteinases in which the preformed active site exists in the latent precursor, the prodomain sterically blocks the active site, and thereby prevents binding of substrates.
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Multiple proteinases have been found in T. vaginalis (Carlton et al. 2007), and many of them have been implicated in the virulence of the parasite (Dailey et al. 1990; Ramon-Luing et al. 2010).
Clearly, the extracts contained multiple proteinase species of varying molecular weight exhibiting gelatinase activity.
Zymography demonstrated elevated levels of proteinases in atheromatous plaques.
Significantly high relative proteinase activity of∼95% was detected in cell-wall fractions and ∼5% activity was observed for osmotic fluids, implying that proteinases in LDL 313 are cell-wall bound.
We have now expressed functionally active recombinant ACP1 (EhCP3) and ACP2 (EhCproteinasesases in baculoviral expression vectors.
As there are 206 annotated chymotrypsin-fold serine proteinases in Drosophila, identification of target proteinases is difficult.
We also tested the effect of various synthetic proteinase inhibitors on 2F5 processing by N. benthamiana proteinases in vitro.
In M. sexta, PAP1 (proPO-activating proteinase 1) and PAP3 (proPO-activating proteinase 3), containing a group 2 clip domain, are terminal proteinases in the cascade pathway and known to be involved in proPO cleavage and activation [ 42, 45].
Lysosomal cathepsins B, L, and D (CB, CL, and CD) are representative cysteine and aspartic proteinases in lysosomes and major proteinases in CNS neurons.
Cysteine proteinases perform multiple functions in seeds, including participation in remodelling polypeptides and recycling amino acids during maturation and germination.
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