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In line with this postulate, Thy-1 exists in a multiple protein complex that contains both Csk and Fyn, a SFK family member [2].
On the contrary, the use of ICAT quantitative proteomics to compare specific and control IP samples (IP-ICAT approach, Figure 3B, right) allows simultaneous detection of multiple protein complex components without using a gel.
This multiple protein complex is crucial in autophagosome formation.
20, 21 Wybutosine synthesis might proceed through sequential reactions in a multiple protein complex assembled with the precursor tRNA and may be linked to exosomal/proteosomal structure that includes the SBDS protein.
To investigate possible protein-protein interaction, multiple protein complex immunoprecipitation (Co-IP) experiments were preformed, but they failed to demonstrate tight binding between tankyrase 1 and DNA-PKcs (data not shown), a negative result that argued against a physical interaction and for an enzymatic one.
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Multiple protein complexes regulate the Rag GTPases in response to amino acids, including GATOR1, a GTPase activating protein for RagA, and GATOR2, a positive regulator of unknown molecular function.
The intrinsically disordered yeast protein Sem1 (DSS1 in mammals) participates in multiple protein complexes, including the proteasome, but its role(s) within these complexes is uncertain.
Current data supports an interaction model where RASSF serves as an adaptor for the assembly of multiple protein complexes and further functional interactions, involving MST kinases and other SARAH domain proteins, which could be regulated by Ras.
Abi1 is a central regulator of actin polymerization through interactions with multiple protein complexes.
It is tightly regulated by a number of autophagy-related genes (ATG) and other multiple protein complexes (Kroemer and Levine, 2008).
Sox2 protein was detected over a size distribution ranging from ∼40 kDa to >800 kDa (Figure 1), arguing that Sox2 is present in multiple protein complexes.
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