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When the precipitate was analyzed by electrophoresis in an 8% native polyacrylamide gel, multiple protein bands were found (Figure 4A).
Sera OB-041, HI-829, and RA-880 reacted with the highest intensity, whereas serum DM-119 reacted against multiple protein bands, including a 75-kDa band, consistent with its atypical DFS staining pattern observed in Fig. 1.
However, analysis of the individual peak fractions by SDS-PAGE revealed the presence of multiple protein bands (Figure 4A & B).
Western blots of wild type animal lysates revealed that the monoclonal antibodies recognized multiple protein bands of 150 kDa, a dimer, and 60 kDa.
The multiple protein bands reacting with antibody against M1 protein are typical of hot acid extracts and were observed for all of the WT and recombinant strains.
Multiple protein bands were detected in blots for TCF7L2, a gene with numerous alternatively spliced transcripts[29], but no mutant proteins were identified that correlated with the mutation data.
Similar(44)
Multiple VEGFR2 protein bands that may correspond to different glycosylated forms of human VEGFR2 expressed by the tumour cells and mouse VEGFR2 expressed by the host endothelial cells were observed in PC3 and A375P tumours.
Like HeLa lysate/Bortezomib/DSS1-biotin mixtures, Fig. 1C (L5) shows that HeLa lysate containing [35S]-labeled DSS1-myc/Bortezomib mixtures produced multiple DSS1-associated protein bands which were SDS-resistant.
Second, we detected the presence of multiple intermediate size protein bands on Western blots of isolated mitochondria but not permeabilized myofibers, indicating partial cleavage of a fraction of the probed proteins into smaller fragments by the protease Nagarse.
In addition, multiple high molecular weight protein bands whose molecular weights were distinct from DSS1 and its oligomers were more prominent when DSS1-biotin was incubated with HeLa lysates (L3) than with DSS1-biotin alone (L1) or HeLa lysate alone (L2).
Multiple O-GlcNAc modified protein bands were observed around expected ∼30 kDa PHB band.
More suggestions(15)
multiple protein sets
multiple protein kinases
multiple protein concentrations
multiple protein isoforms
multiple protein pairs
multiple nonoverlapping bands
multiple protein structures
multiple protein contents
multiple frequency bands
multiple protein domains
multiple protein databases
multiple protein variants
multiple protein aggregates
multiple protein fragments
multiple protein targets
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