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Sequences were aligned using the multiple protein alignment algorithm, Clustal W [41], and analyzed using Philip version 3.5 (DNA parsimony program).
Analysis by PROSITE [67] and multiple protein alignment reveals obvious structural conservation between the characterised human neurexins and fly (Drosophila) and honeybee neurexin I. AmNrxI-A possesses a signal peptide, trans-membrane domain and C-terminal PDZ binding motif, as in the vertebrate neurexins (Figure 4.1 and Figure S4).
The multiple protein alignment of human albumin, alpha-fetoprotein, and afamin was performed using ClustalW (Larkin et al. 2007).
Furthermore, CDD database searches and multiple protein alignment revealed that the S-1 protein contained the consensus motifs of partitivirus RdRps (RNA_dep_RNAP, cd01699).
Additional file 13: A CLUSTALW multiple protein alignment of the AA and AB QTL genotype porcine amino acid sequence compared to bovine, human and mouse GBP5 protein.
MUSCLE or PRANK are currently available for multiple protein alignment, Gblocks [ 65] and a home-made tool for sequence trimming, TreeBest for phylogenetic tree reconstruction (unpublished), and evaluates site and branch-site searches using codeml.
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From multiple protein alignments including sequences identified in the four aerobic bacteria (A.
Multiple protein alignments showed that MnGus protein had homology to the known and predicted Spsb homologues in other animals.
ClustalW [93] multiple protein alignments were used to construct phylogenetic trees as determined by the Neighbor Joining method with bootstrap re-sampling (detailed in the legends for Figures 1), using MEGA3.1 [94].
Several programs use multiple protein alignments to improve the back-translation [ 12, 13].
ClustalX version 2.0.5 [ 46] was used for multiple protein alignments with manual adjustment.
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