Third, neither mutant strain was able to form multiple projections at high concentrations of α-factor.
And her installations have become more elaborate: her most ambitious work to date, "teleco-soup," now representing Japan at the 2011 Venice Biennale (through Nov. 27), uses mirrors, curved walls and multiple-channel projections to give visitors the illusion that they are walking through a city subsumed by floodwaters, blossoming roses and fire.
We interpreted these results with a model in which Ste5p possesses a polarizing function as an early marker (described in the previous section) that in combination with Ste4p can give rise to multiple projections; one role Ste4p may play is inducing the appropriate level of transcription (Figure 9).
To test whether Ste4p was also sufficient in combination with Ste5p to induce multiple projections, we simultaneously overexpressed both AI-Ste5p and AI-Ste4p in the wild-type background.
The morphological patterns were almost identical between AI-Ste5p ste2Δ and AI-Ste5p sst2Δ (Figure 6C), suggesting that the ability to form multiple projections in AI-Ste5p ste2Δ was because of elevated basal heterotrimeric G-protein activity [25], which also led to an enhanced transcriptional response.
These data suggest that a minimum level of transcriptional activation is necessary to form multiple projections.
However, the correlation between transcription levels and the ability to make multiple projections is somewhat loose.
Interestingly, none of the alternative inputs were sufficient to produce multiple projections although some produced a single projection.
Following up this lead, we demonstrated that AI-Ste4p and AI-Ste5p were sufficient to produce multiple projections when combined.
Pursuing this lead, we demonstrated that Ste4p and Ste5p were sufficient to produce multiple projections when overexpressed together.
