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The serum combinations for multiple probing were selected to allow good pattern definition for assigning Western blot spots with protein spots in the silver-stained gel, and are listed in Table 1 where the numbering of the sera corresponds to the numbering in Figure 1.
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Each probe was treated independently and transcripts interrogated by multiple probes were not summarized.
In addition, multiple probes were designed against long PCR fragments generated from phage Lambda.
If multiple probes were present for an mRNA gene, the mean value of all probes was used.
When multiple probes were present for a gene, we selected the probe with the highest variance across the samples.
When multiple probes were present for one gene, we selected the probe with the highest variance across the samples, ending up with 291 unique DASL probes.
Also, in case of genes, for which multiple probes were present, the two different stringencies selected different probes, from which the average values were calculated.
When multiple probes were utilized, each probe was assembled separately and then mixed in SLO/medium just prior to delivery in equimolar concentrations.
The estimated maximal regions of continuous defects affecting multiple probes were identified by the physical positions of the nearest flanking unaffected probe at each end.
When multiple probes were mapped to the same GeneID (EntrezGene identification number), the one with the highest variance in a particular dataset was selected to represent the GeneID.
Where multiple probes were available, expression was averaged.
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