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Exact(5)
For each disorder, gene-gene correlation coefficients within a disorder causing gene list were calculated as described above except that for genes with multiple probesets, the correlation coefficients between the probesets of the same gene were excluded.
For genes identified by multiple probesets, the probeset with the best-performing FDR was selected.
For control genes with multiple probesets, the one with the highest CV was chosen.
For genes matching multiple probesets, the expression pattern was assessed by a "present call" in at least one probeset.
In order to eliminate multiple probesets, the raw data were clustered based on Unigene identifiers (ids), which represent unique transcription loci.
Similar(55)
As these data represent the median values for multiple probesets targeting the length of the gene, it is probable that the RNA degrades along the entire length of the TP63 transcript.
Likewise the multiple probesets for the growth hormone genes, GH1 and GH2, were closely associated within the testicular network graph alongside the chorionic somatomammotropin hormones (CSH1, CSH2, CSHL1) which are all sited at the same loci on chromosome 17.
Furthermore, our method of averaging the signal value of multiple probesets targeting the same transcript on the U133 Plus 2 is only a rough compromise.
For genes with multiple probesets, only the probe set with the largest variance was used [ 23].
The Affymetrix U133A, U133 Plus 2.0 and U95AV2 arrays have different design criteria that may lead to the creation of multiple probesets for the same gene [ 33].
When multiple probesets represented the same gene, we show results for the probeset with the highest call; the results (P values and fold change in expression) are very similar independently of which probeset is used.
Related(20)
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