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Multiple probes aligning to the same gene were averaged together.
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We omitted sites for which multiple probes aligned to the same location.
Sequence matching eliminates errors introduced by mis-annotation, and potential discrepancies introduced by probes aligning with multiple family members or alternative transcripts.
Genome coordinates show the cause of alignment failures, such as probes aligning to an intron.
After alignment to the published flax genome assembly (version 1.0) [ 12], 27 probes aligned to 15 distinct LuBGAL CDS sequences, with multiple probes corresponding to individual genes for added replication.
In cases with multiple probes per gene the most variable probe was selected, resulting in 18,295 unique genes.
In cases with multiple probes per gene the most variable probe was selected, resulting in 18,295 unique coding genes.
Simultaneous imaging of multiple probes or biomarkers represents a critical step toward high specificity molecular imaging.
For genes with multiple probes, the average values of the probes were used.
The rest of the genes represented by multiple probes showed consistent expression patterns among the probes.
In the case of genes with multiple probes, the result for the probe with the greater signal intensity was chosen.
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