Data from multiple primary cultured cells derived from brain suggested a more refined mitophagic response than the near complete loss of mitochondrial material observed in immortalized cell culture systems, but a uniform regulation of Bax by parkin.
The primary cultures were cultured at 37°C in 5% CO2.
This finding underscores the necessity to characterize the expression of possible downstream targets across multiple human glioblastoma lines and primary cultures, and to find those characteristic differences between lines that account for the inconsistencies.
To determine the ex vivo relevance of this putative JAM-A signalling pathway, we sought to verify our results in primary cultures isolated from multiple tissues of patients with breast cancer (Table 1).
In addition, we have studied SAA protein expression and secretion in multiple primary gynaecologic malignancies including USPC and cervical cancer tumour cultures.
Briefly, primary cultures of human fetal RPE cells from multiple donors were trypsinized and resuspended in media supplemented with 10% FBS.
However, the reproducibility of our results across multiple primary cell isolates at each stage indicates that this primary cell culture model has the necessary fidelity for demanding mechanistic studies.
As microglia express multiple TLRs and become activated early during NSV encephalitis (Irani and Prow, 2007; Prow and Irani, 2007), primary cultures of these cells were prepared from wild-type and UNC93b1-mutant animals for in vitro study.
Studies using primary cultures of human endometrial stromal cells (ESCs) have revealed that this process is complex and likely to involve multiple factors including PGE2, relaxin and cAMP in addition to progesterone [4], [7], [8], [9].
For Bachmann, the role model is probably John McCain or even Ronald Reagan's multiple primary campaigns.
