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On this note, it must be highlighted that this study utilized one primary cell line from a healthy donor, and therefore to investigate these findings further one would need to take a more comprehensive approach using multiple primary cells lines from various donors spanning the age and cardiovascular health spectrum.
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Additionally, as regulatory function varies in a cell-specific manner, annotation of multiple primary cell types and careful consideration of the biologically relevant cell types will be required.
However, the reproducibility of our results across multiple primary cell isolates at each stage indicates that this primary cell culture model has the necessary fidelity for demanding mechanistic studies.
10.7554/eLife.03464.006 Figure 4. TNF induces death in multiple primary cell types, and is marked by caspase-3 and -8 cleavage in primary keratinocytes and mouse dermal fibroblasts in vitro.
An additional benefit of using multiple primary cell lines from various donors would be to determine whether our observed findings are applicable to an array of phenotypically diverse fibroblast cells across the spectrum of myofibroblast differentiation.
Data from multiple primary cultured cells derived from brain suggested a more refined mitophagic response than the near complete loss of mitochondrial material observed in immortalized cell culture systems, but a uniform regulation of Bax by parkin.
We tested 309 mostly pesticide active chemicals in 467 assays across nine technologies, including high-throughput cell-free assays and cell-based assays, in multiple human primary cells and cell lines plus rat primary hepatocytes.
Using 467 in vitro assays across 9 technologies, including high-throughput cell-free assays and cell-based assays in multiple human primary cells and cell lines, they investigated a broad spectrum of chemical activities at the molecular and pathway levels.
Importantly, this identifies a consistent diabetic epigenotype of metabolic memory regardless of ulcer status across multiple primary fibroblast cell lines.
In fact, we demonstrated that c-Photina stem cells can be differentiated into two specific cell types and can be used as source of multiple primary-like cells for Ca2+ functional studies.
The factors that determine development of single and multiple primary cutaneous basal cell carcinomas (BCCs) are unclear.
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