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These experiments involve the MS/MS analysis of wide mass ranges resulting in the fragmentation of multiple precursors in a single scan.
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The multiple precursors (2∼6) in some chordates might be a response to complex history of gene duplication with less or no variation in miRNA sequences.
Difficulties arise when multiple precursors are selected resulting in compound MS/MS spectra.
For comparison, the 20-least enriched miRNAs were examined, again discarding those that can arise from multiple precursors, which gave 15 miRNAs in the "depleted" set (enrichment ratios = 0.15 0.74).
We noticed several RNA molecules of 80-200 nt in size that might be the multiple precursors.
As a miRNA can be coded by multiple precursors, the weighted miRNA count attached to each miRNA precursor is reported in "miRNA_expression_raw.xls", with mature and precursor miRNA IDs and hyperlink to miRBase (http://www.mirbase.org/, Figure 2B).
The critical role of the PCs in the proteolytic maturation of multiple precursor substrates implicated in neoplasia makes these enzymes attractive targets in cancer therapy [39].
The multiple precursors might be a response to complex history of duplication with less or no variation in miRNA sequences.
Because some of these miRNAs were derived from multiple precursors, these 172 nov-sit-miRNAs corresponded to 212 pre-miRNAs, 68% of which were located in intergenic regions.
Multiple precursor peptides are present in each pathway, and only 1 or 2 from each set of precursors were selected for study, as designated by the different numbers.
These results for individual charge-reduced precursors showcase the potential benefits of combining ETUVPD results from several charge states or ideally via analysis of multiple precursor charge states at once (as is possible in the HCD cell).
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