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Previously we showed an association at the time of clinical disease between PrPres amyloid and the ISF drainage system using ISF tracers [ 29]; however, in this work we did not look at multiple preclinical time-points to determine the localization and pattern of spread of the PrPres amyloid over time.
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For vCJD, animals were sacrificed at three preclinical time points (132 wpi (n = 1), 144 wpi (n = 1), 158 wpi (n = 1)), and one clinical time point (172 wpi (n = 1)).
In muscle, PrPSc and prion infectivity are uncoupled with detectable PrPSc but no prion infectivity at preclinical time points.
The preclinical time-point for the experimental exposure of VRQ/VRQ group was at 4-10 months after transfusion of weaned lambs.
Here we show that myositis positively influences muscular PrPSc accumulation at preclinical time points and that PrPC-expression in the lymphoid system is critical for this.
We therefore sought an assay that allowed scoring the response at multiple time-points.
If data from multiple time-points were available, the primary end-point data were used.
In addition, several studies evaluated the same patient at multiple time-points with different diagnostic tools.
Screening should include multiple domains and multiple time points before referrals are made to early intervention programs.
A two-way ANOVA was used to analyse multiple treatments over multiple time points.
Two-way ANOVA followed by Bonfferoni post-tests was used for multiple groups with multiple time points.
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