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Genome-wide analysis of re-replication reveals inhibitory controls that target multiple stages of replication initiation.
Limiting levels of initiation factors have been reported [ 15, 16], and constitute a point of control of replication initiation.
Conversely, exemplary LRES regions that replicate later in LNCaP, potentially due to loss of replication initiation zones, are shown (Fig. 4g, Supplementary Figure 10).
Supposing that condensin organizes and separates multiple origins present in nucleoids under rich medium conditions, then artificially reducing the growth rate and thus the rate of replication initiation might suppress the condensin phenotype in rich medium.
Concomitantly, replication origin sequences, which determine the locations of replication initiation events, can affect chromatin structure and modulate transcriptional efficiency.
Beck, H. et al. Cyclin-dependent kinase suppression by WEE1 kinase protects the genome through control of replication initiation and nucleotide consumption.
Numerous mechanisms exist to ensure the appropriate replication of chromosomes, including precise control of replication initiation [1], [2].
Consider multiple points of view.
Nucleosomes influence multiple steps during replication initiation.
We conclude that the loss of a strong origin of replication can be compensated by the activation of multiple weaker replication initiation sites.
Optimization of a TMV vector by removal of putative splice sites and insertion of multiple plant introns increased the efficiency of viral replication initiation by up to three orders of magnitude in N. benthamiana infiltrated leaves [ 49].
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