Sentence examples for multiple point mutations from inspiring English sources

It would also be expected to correlate with the presence of recombination or gene conversion, either of which would be expected to be mis-identified as multiple point mutations in a purely mutational phylogeny.

With recombineering one can make gene replacements, deletions, insertions, inversions, and single and multiple point mutations.

By using two distinct biosynthetic pathways, we demonstrate that DNA assembler can perform multiple tasks, including heterologous expression, introduction of single or multiple point mutations, scar-less gene deletion, generation of product derivatives, and creation of artificial gene clusters.

We present a robust and simple method for the direct detection of multiple point mutations in the Mycobacterium tuberculosis rpoB gene during the development of rifampin (RIF) resistance using an electrochemical genosensor.

To determine the critical residues in CTCF binding site that are required for both M1 and M2 to bind to CTCF, we have looked into CTCF binding motif M1 and M2 and designed multiple point mutations according to the position weight matrix score (Schmidt et al., 2012).

While genetic characterization of HTM using 127 markers, at an average distance of one marker per 3 Mb, revealed a very high degree of genetic similarity with the WT, Nagina 22, the causal gene sequencing results indicated multiple point mutations in HTM resulting in eight amino acid changes in a distance of just 1.88 kb (Fig. 5).

The preliminary identification of the wheat EPSPS-AB and EPSPS-D cDNA sequences allowed the design of genome-specific primers using sequences containing multiple point mutation differences (Table  1; Additional files 1 and 3).

In order to explore whether Ag85A DNA vaccine could mount more efficiently protective immune response through new RNA sensor and its signal transduction pathway of antigen presentation we designed and synthesized Ag85A gene fragment containing multiple points mutations and transfected the gene fragment into the dendritic cell line (DC2.4) by CRISPR/Cas9.

One-, two- or multiple-point mutations are possible by specification of residues to be mutated and the required substitutions.

In general, neither the original RNAMute [ 41] nor RDMAS [ 42] can handle multiple-point mutations.

To determine whether Mto1 can localize to SPBs independently of eMTOCs, we generated a series of single- and multiple-point mutations within the portion of MASC required for eMTOC localization in the context of full-length Mto1.