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Importantly, of the 96 new isolates analyzed, 30 had multiple ploidy subpopulations and 21 of these contained a detectable haploid subpopulation, supporting the hypothesis that haploids appear frequently, albeit transiently, under conditions selecting for loss of three allelic markers.
A significant proportion of the passaged cultures had not resolved to a single ploidy by day 28 and instead had multiple ploidy subpopulations in the culture (22% initially tetraploid, 16% initially aneuploidy; Figure S4), identified by multiple G1/G2 peaks in their flow cytometry profile (for examples see Figure 2B and description in Hickman et al. 2013).
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Like many perennial plant species, A. gerardii exists in populations with multiple ploidy levels [50].
Multiple ploidy levels per species [37], hybridization and polyploidy [17], [38], [39], [40], [41], and DNA content [20], [42] have all been linked to invasiveness in previous studies.
Tall fescue exhibits multiple ploidy level variants from tetraploid to decaploid [ 9, 10].
Festuca rubra is perennial, rhizomatous, and highly interfertile with multiple ploidy levels (Dirihan et al., 2013).
Other examinations of SRAP diversity in taxa with multiple ploidy levels have also found associations with fragment diversity and ploidy (Gulsen et al., 2009).
Rangewide determination of DNA ploidy in A. senegal found diploids, triploids, tetraploids and a hexaploid including some populations with multiple ploidy levels (2 x + 4 x, 2 x + 3 x and 2 x + 6 x).
The input file was prepared accordingly to multiple ploidies [ 32] with adaptations.
The second approach is to apply cooperative coevolution technique to coevolve multiple subpopulations for multiple decision rules.
LSC activity was identified in multiple HSPC subpopulations suggesting there is no canonical LSC immunophenotype in human t-AML.
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