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Insoluble nature of these constructs permit highly efficient, multiple plasmid modification reactions thereby presenting new paradigm in reusable chemical nuclease design.
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We evaluated multiple plasmid designs for IL-12 expression.
The construct with multiple plasmid copies of ORF1 had an approximately 10-fold increase in levels of nicotinamidase activity.
Cells transfected by this method usually carry multiple plasmid copies inserted in tandem in the genome.
Cells might acquire multiple plasmid copies in several ways.
Correct orientation was determined by sequencing multiple plasmid clones.
The notion is applicable to nine plasmids from six major incompatibility groups and mixed populations carrying multiple plasmids.
The method described here could also be particularly useful in curing individual plasmids from strains that carry multiple plasmids.
These organisms possess multiple plasmids.
Many bacterial strains contain multiple plasmids, but the relationships between bacterial plasmids and chromosomes are unclear.
All 4 isolates contained multiple plasmids (data not shown).
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