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This drug is activated after a series of multiple phosphorylation events when it is converted to AraC-triphosphate [210, 211].
Importantly, multiple phosphorylation events on a clock protein, presumably by multiple kinases appear to be a common mode of regulation.
Cortactin is regulated by multiple phosphorylation events, including phosphorylation of S405 and S418 by extracellular regulated kinases (ERK 1/2.
We have developed a protocol that utilizes the multi-parametric and quantitative attributes of FACS to examine multiple phosphorylation events at the single cell level in highly purified HSC/HPC populations.
One explanation for this could be that the hyperphosphorylated isoform contains multiple phosphorylation events between Ser14 and Ser288 and that the absence of a single one of them does not alter the isoform mobility.
Multiple phosphorylation events were observed on each array (data not shown).
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This model predicts that multiple FRQ phosphorylation events contribute to FRQ stability.
In the present study, we found that ERK1/2 undergoes multiple temporal phosphorylation events in response to TNFα-induced MEK1/2 activation.
The kinase activity of CTR1 is reported to be regulated by multiple reversible phosphorylation events, leading to significant conformational rearrangements [ 41].
The most thoroughly studied of these is 4E-BP1, for which multiple and hierarchical phosphorylation events are required for release from eIF4E [26].
These include multiple phosphorylation and acetylation events, glycosylation, ubiquitylation and sumolyation amongst others (reviewed by Martin et al., 2011).
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