Random mutagenesis of E. coli primary σ70 factor was found to result in global perturbations of the transcriptome and the mutants exhibiting ethanol tolerance, increased lycopene production and multiple tolerance phenotypes, respectively, were obtained (Alper and Stephanopoulos 2007).
A total of 163 nontandem duplicate genes that were related to 75 kinds of phenotypic change were classified into 26, 89, 32, and 16 genes whose multiple-knockout mutants showed phenotypic changes in the seed, vegetative, reproductive, and conditional phenotypes, respectively.
No matter how optimized the GLS solver, such an approach is prohibitively expensive for either single or multiple phenotypes, due to the unmanageable complexity of O(mn ) and O tmn ), respectively.
In this study, two metabolically distinct breast cancer cell lines were utilized, BT-474 and MDA-MB-231, representing mitochondrial and glycolytic phenotypes, respectively, and nontumorigenic breast epithelial cell line, MCF-10A, to identify the efficacy of DHA in multiple metabolic pathway.
It probably has multiple phenotypes," he says.
In addition, none of existing methods can handle multiple phenotypes.
Asthma is a heterogeneous disease with multiple phenotypes.
Results from three distinct phenotypes (ethanol tolerance, metabolite overproduction, and multiple phenotypes) are provided as proof-of-concept.
Using these technologies, identifying differentially expressed genes (DEGs) among multiple phenotypes is the very first step to understand difference between phenotypes.
Macrophages can exhibit multiple phenotypes and are known inflammatory regulators in many tissues, but their phenotypes have not been characterized in IVD degeneration.
