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For seven proteins multiple peptides were tested.
For 16 blastocoels, multiple peptides were successfully detected (3.3 proteins per sample on average).
Only proteins in which multiple peptides were identified were considered positively identified proteins.
At this threshold, spectral counts for multiple peptides were observed in most sample replicates, thus suggesting sustained production under the range of growth conditions tested.
Only proteins in which multiple peptides were identified or in which single peptides were identified in multiple animals were considered positively identified proteins.
One such promising biomarker was leucine-rich alpha-2-glycoprotein (LRG1), where multiple peptides were found in all urines from OvCa patients, but only one peptide was found in one healthy control urine sample.
Similar(52)
Consequently, the identification of multiple peptides is not due to shared fragments between peptides.
This process is fully additive, and gradients of multiple peptides are easily achieved.
The fact that many of these peptides change levels as a function of time of day suggests that multiple peptides are likely to have roles related to timekeeping, feeding or energy homeostasis.
Results showed that strong cross-reactive responses to multiple immunodominant peptides were associated with better clinical outcomes.
Out of the multiple Leishmania proteins to which the tryptic peptides mapped only those proteins where multiple peptides could be assigned are shown (Figure 6C).
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