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The results of this study further substantiate the concept that rational design and testing of multiple peptides for the same T-cell epitope should elicit a broader response among different individuals than single peptide immunization.
Multiple peptides for β-subunit Os05g41220 were also associated with SnRK1 α-subunit Os05g45420 (Table 1, no. 12).
Analyses of TAP-tagged PKs that recovered only a single peptide for potential interacting proteins or multiple peptides for unique isoform members of a family of abundant proteins are shown in Supplemental Table S1-B.
A second purification of each complex found multiple peptides for β-subunit Os03g20340 which were associated with both SnRK1 α-subunit Os08g37800 (Table 1, no. 11) and SnRK1 α-subunit Os05g45420 (Table 1, no. 12).
The selection and use of multiple peptides for a target protein is useful for increasing the credibility of the absolute quantification.
Within just 2 3 years, multiple peptides for each of the expressed yeast genes and now 99% of the 20,300 human gene-coded proteins have been prepared; their mass spectra have been determined and shared publicly through the SRM Atlas.
Similar(54)
Peptides were predicted by FrameDP, which often produces multiple peptide for a single unigene elements.
Other positive indicators include: 1. the appearance of the partner in at least 50% of the top cohort (i.e., first 10 matches) of any one search; 2. the appearance of the same or related hits occurring in several different searches; 3. the identification of the same partner for multiple peptides from the same or related pans.
However, sequential NCL with multiple peptides allows for the preparation of fully synthetic histones, where multiple distinct histone PTMs can be incorporated throughout each histone.
Indeed, we detected multiple peptides unique for the testis-specific expressed gene 1 protein (H2A-Bbd), an atypical histone H2A variant associated with active transcription and mRNA processing [see Additional file 2: Table S1].
Furthermore, some compounds obtained excellent DS for multiple peptides.
Related(20)
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