With further improvements of next generation DNA sequencing techniques and further reductions of sequencing costs, it may be feasible to extend this study to analyze the transcriptomes of RASFs isolated from multiple patient samples at progressing stages of pathogenesis.
Multiple blaTEM variants were suggested to coexist among 3 clinical isolates indicating extensive role of TEM β-lactamases for resistance against cephalosporins in our patient isolates.
From this collection of strains 153 S. Virchow strains (1,5%) from different patients (multiple isolates from same patient excluded, but family members not excluded) were selected and integrated in this study.
We identified 673 isolates that belong to as-yet-undescribed species, including 348 isolates of 95 novel taxa that were isolated from multiple patients.
In this case the predominant alleles in C. albicans populations colonizing a given host would continually change [20], [22] and we would not expect to find alleles predominating in collections of isolates from multiple patients.
The use of genotyping methods has identified >100 V. vulnificus strains in a single oyster (4 ) and notable heterogeneity among clinical isolates from multiple patients, even if a unique pathogenic strain causes the infection in each patient.
Rapamycin treatment was also associated with decreased proliferation of NB TICs isolated from multiple patients (Fig 5B).
Isolates from 95 novel taxa were recovered from multiple patients, indicating possible clinical relevance.
Neimann et al. saw almost no change in patterns among 75 isolates in chains of transmission, i.e., multiple patients (30).
This results in a complicated situation, because transmission will not only be reduced after detection, the contact structure will also change, with only a limited number of people (health-care workers) having contact with multiple isolated patients.
