All randomly selected A95 transformants maintained hygromycin resistance after multiple passages on MEA with or without hygromycin.
While there are some indications in the data above that Bcl-XL is more efficient than Bcl-2 at inhibiting apoptosis, quantitative comparison of Bcl-2 and Bcl-XL requires selection of transfected clones stably expressing known amounts of protein that does not vary with multiple passages, unlike what can be observed with pools of transfectants used in previous studies.
Each chapter of "Reading Jesus" begins with multiple passages from the Gospels, chosen for what they reveal about Jesus' teachings and how they compare in different tellings.
I came back the second week with my copy of The Count of Monte Cristo marked up with multiple passages I didn't understand.
Briefly, the cells were harvested by scraping into homogenation buffer (200 mM sucrose, 20 mM Tris [pH 7.4], 1 mM EGTA, 1 mM EDTA, 1 X complete protease inhibitor cocktail), lysed by multiple passages through a syringe with a 26-gauge needle, and ultracentrifuged at 70,000×g for 30 min at 4°C.
The established cell lines also undergo genomic changes with multiple passages in culture [ 9- 11].
By routinely propagating lines at high density and passaging with trypsin, we can maintain cell lines with normal karyotype for multiple passages.
Interestingly, the piPSCs contained abundant episomal vectors and maintained high-level transgene expression even after multiple passages in the N2B27 medium supplemented with small molecules (Fig. 2C and Fig. S2B), suggesting a likely mechanism that these small molecules improved episomal reprogramming was through the retention of episomal vectors and transgene expression.
These Esrrb null ESCs remained morphologically undifferentiated in serum-containing medium with LIF over multiple passages.
The cultured MB468 cells at early passages typically appear like epithelial cells with oval and/or polygonal shapes [ 16]; and after multiple passages (50+), these cells exhibit apparent mesenchymal phenotypes with spindle and elongated shapes [see Additional file 1], which are ideal for the proposed studies.
