Each chapter of "Reading Jesus" begins with multiple passages from the Gospels, chosen for what they reveal about Jesus' teachings and how they compare in different tellings.
For instance, multiple passages of the cell lines IMR90 (human fetal lung fibroblast) and H1 (human embryonic stem cells) showed high concordance [ 17].
Additionally, 11 genomic replicates were sequenced for the validation experiment, including multiple colonies from the same plate (n = 4), multiple passages of an isolate (n = 4), and independent sequencing experiments (n = 4) from the same DNA source.
This process is repeated through multiple passages to allow for expansion to occur.
Engineered vector constructs were examined for replication, encapsidation, GFP expression during multiple passages in protoplasts, and for their ability to infect, move, express GFP, and be maintained in citrus plants.
It seemed justified that if these cells were to survive and propagate in culture for multiple passages (over 30), high levels of LC3 II (signifying abnormal autophagy) could be detrimental for the cells.
This was achieved by serially passaging viable cells for multiple passages.
Similar to "good" preparations of manually isolated NSC, these cultures were often free of NSC contaminants and could be cultured in vitro for multiple passages (up to 22 tested).
hES cells (approximately 2×106 cells) grown under animal-derived extracellular matrices-free culture conditions for multiple passages with occasional freezing and thawing were subcutaneously injected into severe combined immunodeficient (SCID / beige mice (Charles River Laboratories, Wilmington, MA).
By routinely propagating lines at high density and passaging with trypsin, we can maintain cell lines with normal karyotype for multiple passages.
