Sentence examples for multiple passages and from inspiring English sources
The amniotic fluid (AF) is an invaluable source of organ-specific progenitor cells and stem cells that are easily obtainable, expandable over multiple passages, and can be used in various regenerative medicine applications.
The heterogeneity in MCF7S seems to be rigidly maintained, as the proportion of self-renewing cells remains almost constant over multiple passages and after antiestrogen treatment (Figure 6B).
Additionally, lines were karyotypically normal after multiple passages and have been propagated for over 30 passages in culture while retaining a normal karyotype (Figure S3).
These ER-positive tumors retain their hormone receptor status over multiple passages and their dependence on estrogen for growth.
The preceding results have illustrated the successful 'hands-free' expansion of mESCs over multiple passages and the maintenance of high levels of cell viability and pluripotency gene expression.
Generating homozygotes in F1 transgenics using a "mutagenic chain reaction" (Gantz and Bier 2015), would limit the need for multiple passages and mating to establish a line.
Limited cell number presents further constraints, particularly for autologous transplantation, as the number of cells per area on tissue culture plates requires multiple passaging and potential loss of pluripotency [ 6].
Engineered vector constructs were examined for replication, encapsidation, GFP expression during multiple passages in protoplasts, and for their ability to infect, move, express GFP, and be maintained in citrus plants.
The presence of a functional spermatogonial stem cell compartment was verified by in vivo spermatogenesis colony forming assays in which genetically tagged donor spermatogonia were thawed from cryo-storage, propagated over multiple passages in culture, and then transplanted into seminiferous tubules of busulfan-treated, DAZL-deficient ratesteses (Fig. 2).
However, the correlation between VHEV and Vilyuisk encephalomyelitis is still uncertain because VHEV has been isolated by multiple passages in mice and thus may represent a highly divergent strain of TMEV.
To minimize possible phenotypic changes in gCSCs due to prolonged maintenance in vitro and multiple passages, early passage gCSCs were frozen in neurosphere medium containing 5% DMSO and stored at −70°C.
