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Several mechanisms are known to overcome these challenges, including the utilization of multiple origins of DNA replication and an elaborate post-replication repair system [2].
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Our studies focus on the events that occur at the starting points of chromosome duplication, called origins of DNA replication.
Origins of DNA replication are marked by the formation of multi-protein complex, called the preRC.
These sites defined the locations of origins of DNA replication.
The preRC marks origins of DNA replication and controls activation of bidirectional DNA replication from these origins once S-phase is initiated.
Furthermore, Gcn5p physically associates with origins of DNA replication, while its deletion leads to localized condensation of chromatin at origins.
Next, we used ChIP assays to test if Gcn5p directly associates with origins of DNA replication.
By determining the sites showing nascent DNA enrichment, we have localized several thousand origins of DNA replication.
The firing of eukaryotic origins of DNA replication requires CDK and DDK kinase activities.
Strains of Escherichia coli diploid for the chromosomal origin of DNA replication.
Remarkably, the silencer also functions as a chromosomal origin of DNA replication, and we have discovered that ORC, the eukaryotic replication initiator, PCNA and other replication-related proteins play critical roles in silencing.
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