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Double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful reverse genetics tool to silence gene expression in multiple organisms, including plants, nematodes and insects.
Cultures from this ulcer revealed multiple organisms including Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa and Proteus mirabilis.
Based on comparative anatomical studies in multiple organisms including telosts [1], we attribute the anterior GFP-expressing cells as those of the diencephalic choroid plexus (dCP), and the posterior GFP-expressing cells as those of the myelencephalic choroid plexus (mCP) [Fig. 1E (lateral section), Fig. 1F (transverse section)].
The IGF-I paradox lies in the fact that despite the proliferation- and survival-enhancing properties attributed to IGF-I, it is a reduction in IGF-I signaling that has been shown to extend lifespan in multiple organisms including nematodes, flies, and mammals [15] [21].
Perhaps HSP is a "final common pathway" of infection with multiple organisms including Bartonella.
IMP provides gene-gene functional relationship networks from multiple organisms including zebrafish.
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Recent single-cell RNA sequencing efforts have defined the transcriptomes of cell types in multiple different organisms, including multiple mouse cell types as an example vertebrate56,57.
CR or intermittent fasting are known for their wide life-span-extending and health-improving effects that can be measured in an objective fashion in multiple model organisms including rodents and primates.
Pharmacological or genetic manipulations designed to prolong lifespan induce autophagy in multiple model organisms, including yeast, nematodes and flies, and the inhibition of autophagy often (always?) prevents longevity extension in such settings.
Moreover, given the increasing popularity of RNAi techniques and rapidly accumulating protein-protein interaction data in multiple model organisms, including human, the applicability of our approach to other species is very promising.
Age-associated neurodegenerative disorders including Alzheimeŕs and Parkinsońs disease may be postponed or attenuated by chronic induction of autophagy, and there is substantial evidence that genetic or pharmacological induction of autophagy can increase the healthspan and lifespan of multiple model organisms including yeast, worms, flies and mice.
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