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In prokaryotes, translation of multiple open reading frames in a polycistronic RNA occurs through multiple independent translation initiations.
In three other cases, a single TCS gene is predicted to be split into multiple open reading frames (ORFs).
Our computational approach accounts for multiple open reading frames, truncated predicted ORFs, and the presence of N-terminal signal peptides, and may be useful for MS/MS-based protein discovery in any species for which EST data are available.
The complete genomic sequences (excluding the 5′- and 3′-terminal segments) of YN isolate comprised 27,635 nt, which putatively contains 6 different genes, each containing single or multiple open reading frames.
For each GO term and PANTHER family, the number of matching tomato and potato BESs was counted; if a single GO term or PANTHER family was assigned to the same sequence multiple times, for example due to multiple open reading frames in the same sequence, it was only counted once.
These properties include (i) the genome organization with multiple open reading frames (ORFs), (ii) the predicted proteomes (Fig. 1), (iii) the production of enveloped, spherical virions, and (iv) the synthesis of genome-length and subgenome-length viral RNAs in infected cells [ 6, 7].
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In turn, activated Gcn2 phosphorylates and thereby inactivates the translation initiation factor eIF2α, resulting in a lower level of general translation and preferential translation of a small subset of mRNAs (such as the GCN4 mRNA), which feature multiple short open reading frames via which translation is regulated [22].
Some annotations contain polycistronic transcripts encompassing multiple independent open reading frames.
In Arabidopsis, carboxyl-terminal truncation alleles of eIF3h cause under-translation of specific mRNAs, many of which harbor multiple upstream open reading frames (uORFs) in their 5' leader [ 8, 12].
The sigma virus P-like NIRV is present in multiple retroelement fused open reading frames on the X and 3R chromosomes of Drosophila yakuba – the X-linked copy is transcribed to produce an RNA product in adult flies.
The pup gene is usually preceded by the dop gene, while the Pup ligase gene, pafA, is located downstream of the proteasomal subunit genes, in some cases separated by multiple functionally unrelated open reading frames.
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