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In the same way, novel multiple nucleotide variants (MNV), frame-shift mutations, and nonsense mutations were detected in each strain (Table 2).
Since multiple nucleotide variants have been described at most of these sites, the test can detect 120 previously described mutations (Supporting Information Table S1).
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In another study, comparison of PDXs at different time points detected multiple single nucleotide variants, but these mostly localized within the noncoding regions, suggesting that they arose over time during passaging [ 49].
As our sequence analysis shows that EBV miRNAs exist with multiple length and nucleotide variants, we decided to use the total read, including all length and nucleotide variants, to represent the abundance of individual EBV miRNAs in NPC tissue.
Whole-exome-sequencing of these 16 resistant clones and comparison to parental cells uncovered multiple unique single nucleotide variants (SNVs) in each resistant clone.
In the AKAP7 long form splice variants, multiple nucleotide mutations and insertions-deletions have resulted in the concurrent loss of AKAP7γ and formation of AKAP7δ in rats.
In addition, predicted genes, single nucleotide variants (SNVs), multiple types of RNA sets and repeats contained within the YGD can be visualized using Gbrowse.
Database search and multiple nucleotide sequence alignment of both the variants showed their conservation across various species (Additional file 2).
Zong et al. [ 35] required data from multiple MALBAC-amplified single cells to detect reliable nucleotide variants.
This shift in the presence of single nucleotide variants suggests the presence of multiple clones rising and falling in dominance over time.
There is no direct evidence for multiple hits on the Cave Bear dataset (no site with more than two nucleotide variants), which is probably due to the high transition transversion bias thereby making such multiple mutations on a site not readily apparent.
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