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ML and BI trees inferred from the multiple nuclear gene sequences generated identical tree topologies.
Unique taxonomic status for the PNT was confirmed through comparison of multiple nuclear gene sequences, and also supported by evidence from chemotypic diversity.
This identifies a smaller set of lineages that can also be tested independently for species status with multiple nuclear gene approaches and other phenotypic characters.
However, species in which well differentiated reciprocally monophyletic clades of COI haplotypes were detected would seem to be fertile ground for further investigation with independent multiple nuclear gene trees in a coalescent framework.
On the basis of the multiple nuclear gene sequences, Eizirik et al. (2010) proposed a complete molecular phylogeny for 50 different genera representing all carnivoran families and constructed a molecular timescale for the evolution of Carnivora.
In this study, we analyzed multiple nuclear gene sequences of eight species and subspecies of Pseudorasbora, Pseudopungtungia, and Pungtungia residing in China, Japan, and Korea to elucidate their molecular phylogenetic relationships.
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In such cases, the analyses of multiple nuclear genes can be more powerful for deciphering interspecific relationships.
These results indicate that in erythroblasts, mitochondrial oxidative stress reduces expression of multiple nuclear genes encoding components of the respiratory chain, TCA cycle and mitochondrial protein synthesis.
Herein, we made use of the phylogenomic approach and available resources via D. rerio to sample multiple nuclear genes and incorporated additional data from Genbank to reconstruct the long-standing problematic phylogenetic relationships of the endemic cyprinids in East Asia.
This result also was supported by two recent studies analysing multiple nuclear genes [ 17, 51].
It remains true that future studies using multiple nuclear genes are desirable to confirm our findings.
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