Table 3 ANA-reflex test procedure with patterns related to markers found in autoimmune liver diseases ANA-IIF pattern on HEp-2 cells Reflex test(s) Cytoplasmic reticular/AMA ≥1 160 Anti-mitochondrial M2 or E3 or MIT3 Multiple nuclear dots ≥1 160 Anti-Sp100 Nuclear envelope Anti-gp210 Cytoplasmic linear-actin ≥1 160 IIF on kidney, stomach, and liver, to confirm anti-actin antibodies.
The profile detected in breast cancer sera showed distinctive features, such as antibodies targeting mitochondria, centrosomes, centromeres, nucleoli, cytoskeleton, and multiple nuclear dots.
With this software, the following six main patterns can be detected readily on HEp-2 cells: cytoplasmic, homogeneous, speckled, nucleolar, centromere, and multiple nuclear dots.
This serum also showed multiple nuclear dots [MNDs], a combination which is thought to be characteristic of PBC [ 21, 23, 33].
Based on the immuno-morphological staining pattern, samples were categorized into three groups: 1) ANA-negative, 2) homogenous ANA ± other ANA patterns, and 3) other ANA patterns: speckled, centromeric, nucleolar or multiple nuclear dots.
Nuclear and cytoplasmic fluorescence including homogeneous, fine and coarse speckled, anti-centromere, and AMA patterns as well as centrosome/spindle apparatus, multiple nuclear dots [MNDs], and cytoskeletal fluorescence were read by three independent observers [CP, FFM, and ML] who were in agreement in more than 97%.
(i.e. with a multiple nuclear dot or a rim like membranous pattern) [ 2].
In addition to AMA, two distinct anti-nuclear antibodies (ANA) patterns are detectable by indirect immunofluorescence (IIF), the multiple nuclear dot (MND) and rim-like (RL) patterns, for which the reactants are sp100 and the glycoprotein gp210 respectively [ 15, 16].
Nanoscale NMR spectroscopy and imaging of multiple nuclear species.
We are poised on the brink of a new age of multiple nuclear powers.
Each had multiple ICBMs with multiple nuclear warheads and separate reentry vehicles.
